ASSOCIATION OF GSTM1 AND PARAOXINASE-1 (L55M & Q192R) POLYMORPHISMS WITH CHRONIC ORGANOPHOSPHORUS INTOXICATION IN EGYPTIAN PESTICIDE APPLICATORS.

Document Type : Original Article

Authors

1 Lecturer in Molecular Diagnostics and Therapeutics Department, Genetic Engineering and Biotechnology Research Institute, Sadat City University.

2 Lecturer in Molecular Diagnostics and Therapeutics Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City

3 forensic and toxicology department, faculty of medicine, menoufia university

Abstract

ABSTRACT
Background: On the work, farmers are more likely to come into contact with pesticides and other agricultural contaminants. The permanent inhibition of acetylcholinesterase (AChE) at nerve synapses is thought to be a systemic impact of organophosphates (OPs). However, several investigations have shown that AChE inhibition alone does not account for all toxicological effects associated with long-term OP exposure. Although oxidative stress and epigenetic modifications are two of the most recently hypothesised mechanisms, the existence of genetic variations appears to have the greatest influence on biological outcomes. Aim: The purpose of this study is to investigate into polymorphisms in genes that code for OPs metabolising enzymes. Because the glutathion-S-transferase (GSTM1) and paroxonase (PON1) genes encode enzymes that breakdown pesticides with carcinogenic potential, we're particularly interested in them. Methods: During the months of June to December 2018, a cross-sectional study was done on 24 pesticide applicators and 25 corresponding controls. Serum AChE activities were determined using the EQM Test-Mate kit. Using polymerase chain reaction-restriction fragment length polymorphism, the influence of genetic polymorphism in GSTM1 and PON1 on OPs exposure were examined. Results: In compared to the control group, chronically exposed patients had significantly lower serum AChE enzyme activity (<0.001). In terms of the GSTM1 polymorphism and the R allele of the PON1 (Q192R) polymorphism, there was a significant difference between chronic OP exposed cases and controls. The PON1 (LM) polymorphism was found to be of little of consequence. Conclusion: Finally, the GSTM1 and R allele of PON1(LM) polymorphisms may be a risk factor with chronic organophosphates intoxication in Egyptian pesticide applicators.
Keywords: Organophosphates; Acetylcholinesterase; Polymorphism; Glutathion-S-transferase (GSTM1) gene; Paroxonase (PON1) gene; Polymerase chain reaction-RFLP.

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