Document Type : Original Article
Authors
1
physiology department, faculty of medicine, menoufia university
2
Medical Physiology Department, Faculty of Medicine, Menoufia University
3
Medical Biochemistry and Molecular Biology Department, Faculty of medicine, Menoufia University
4
Anatomy and Embryology Department, Faculty of Medicine, Menoufia University
5
Clinical Pharmacology Department, Faculty of medicine, Menoufia University
6
Psychiatry department, Sulaiman Alrajhi University, Al Bukayriyah, Saudi Arabia. Psychiatry department, faculty of medicine, Assiut University, Assiut, Egypt.
7
Anatomy and Embryology Department, Faculty of Medicine, Benha University
8
Forensic Medicine and Clinical Toxicology department, Faculty of Medicine, Menoufia University
9
Medical Physiology Department, Faculty of Medicine, Suez Canal University, Ismailia
Abstract
Background: Rats' memory is hampered by the muscarinic receptor antagonist scopolamine (Scop), which inhibits cholinergic neurotransmission. Objectives: to assess the protective impact of sitaglibtin and its underlying processes in neurocognitive impairments brought on by Scop. Methodology: Thirty adult male albino rats were divided into three groups: control, Scop, and Scop+STG (10/group). Rats were evaluated neurocognitively. The following were measured: BDNF, Nrf2, HO-1, citrate synthase (CS) activity, acetylcholine, MDA, SOD, TNF-α, interleukin (IL)-6, IL-10, and amyloid β 42 (Aβ42). Additionally, hippocampal histological and immunohistochemical analyses were performed. Results Along with significantly greater levels of MDA, TNF-α, IL-6, acetylcholine, and Aβ 42, as well as GFAP and Caspase-3 hippocampus immunoreaction, the Scop group also showed a decline in cognitive performance compared to control. Additionally, it down-regulated BDNF, Nrf2, and HO-1 gene expression in the hippocampus and markedly decreased IL-10, SOD, and CS activity. The Scop+STG showed a considerable amelioration in the neurocognitive deficits caused by Scop. Conclusion: In addition to up-regulating Nrf2 and HO-1 gene expressions, sitagliptin has a beneficial protective impact against Scop-induced cognition impairments through anti-oxidant, anti-inflammatory, enhanced mitochondrial activity, prevention of apoptosis, and neurotrophic actions.
Keywords
Main Subjects
)
View on SCiNiTO